Regulatory

Part:BBa_K123003:Design

Designed by: Jason Gardiner   Group: iGEM08_University_of_Alberta   (2008-10-27)

ER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 24
    Illegal NgoMIV site found at 902
    Illegal AgeI site found at 870
    Illegal AgeI site found at 1841
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is evidence that ER can be toxic to cells. However we found that while it does effect cell growth rate the toxicity was not lethal shown in Figure 1.

We also have to condsider that normally when ER forms a homodimer it is transported into the nuclease. However ER will not bind to the Estrogen Receptor Element unless it has been dimerized.


Source

This Part originates as the Human Estrogen Receptor Alpha

References

Please see the 2008 University of Alberta Team wiki